Sex in zeist

Added: Wilfredo Noah - Date: 11.03.2022 02:48 - Views: 45399 - Clicks: 3393

In vitro cultures of intact chick gon organ cultures and reaggregation cultures of dispersed gonad cells roller cultures were made. Gon or gonad cells from 7-day-old chick embryos, at the stage when sex-specific differentiation begins, were cultured in the presence of pd H-Y antigen-containing supernatants, or co-cultured in the presence of H-Y antigen-producing cell lines.

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The H-Y antigen-producing cells tested were of human, mouse, bovine Sex in zeist chicken origin. During organ culture, addition of supernatant of the human lymphoma cell line Daudi, or co-culture with Daudi cells, stimulated a clear proliferation of the germinal epithelium in male gon, indicating feminization. A similar effect was obtained by treatment with estradiol. In reaggregation culture, the increase in nuclear size of germ cells was chosen as a parameter for feminization.

A ificant increase of germ cell nuclear size was observed in gon cultured in the presence of Daudi supernatant. In both organ cultures and reaggregation cultures, other tested H-Y antigen sources and semi-purified H-Y antigen fractions did not exert ificant effects on differentiation of the gon or on the average area of the germ cell nuclei. These findings suggest that it is not H-Y antigen, but another protein produced by Daudi cells, that might be responsible for the sex-reversing effects.

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RSS Link Copy. Cite Display options Display options. Abstract In vitro cultures of intact chick gon organ cultures and reaggregation cultures of dispersed gonad cells roller cultures were made. Similar articles An in vitro model of gonad differentiation in the chicken. Estradiol-induced sex-inversion in the occurrence of serological H-Y antigen.

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Ebensperger C, et al. PMID: A substance secreted by rat Sertoli cells induces feminization of embryonic chick testes in vitro. Anat Embryol Berl. Effect of tamoxifen on H-Y antigen expression and gonadal development in chicken embryos.

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Koo GC, et al. Primary sex determination: genetics and biochemistry. Hall JL, et al. Mol Cell Biochem. PMID: Review. Phenotypic manifestations during the development of the dominant and default gon in mammals and birds. Mittwoch U. J Exp Zool. See all similar articles. References Nature. MeSH terms Animals Actions.

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Cattle Actions. Cell Line Actions. Chick Embryo Actions. Female Actions. Humans Actions. Male Actions. Mice Actions. Organ Culture Techniques Actions. Tumor Cells, Cultured Actions. Substances H-Y Antigen Actions. Estradiol Actions. Copy Download.

Sex in zeist

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